- If extraction efficiency is 80%: ``` Adjusted raw material = 4 g / 0.8 ≈ 5 g ```
Consider Solvent Volume and Concentration
- Suppose you want a final concentration of 50 mg/mL in your solution. - Total volume needed: 200 mg / 50 mg/mL = 4 mL. - You would dissolve the extracted material into 4 mL of solvent.
Final Preparation Steps
- Mix thoroughly, ensure complete dissolution (use sonication if needed). - Filter to remove any insoluble particles. - Store under appropriate conditions (e.g., at 4 °C, protected from light).
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Practical Tips & Safety
Task Recommendation
Handling of acids/bases Use a fume hood; wear gloves and eye protection.
Filtration Use a filter that matches the pore size required (e.g., 0.45 µm).
Solvent evaporation Perform under reduced pressure to avoid overheating.
Storage Protect solutions from light if the compound is photosensitive.
Documentation Record all volumes, concentrations, and observations in a lab notebook or electronic log.
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Summary
Prepare an aqueous extraction solution (adjust pH as needed).
Extract the desired component into this phase by mixing and allowing it to partition.
Isolate the extract via filtration or centrifugation, concentrating if necessary.
Purify further using chromatography or crystallization depending on purity requirements.
With these steps you can effectively isolate a compound from an aqueous mixture, achieving a high yield of pure product ready for downstream applications.
